Synthesis of ethyl isovalerate using Rhizomucor miehei lipase: optimization.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/2070/
|
|
| Title |
Synthesis of ethyl isovalerate using Rhizomucor miehei lipase: optimization.
|
|
| Creator |
Chowdary, G. V.
Prapulla, S. G. |
|
| Subject |
04 Fungi
05 Enzymes |
|
| Description |
Immobilized lipase from Rhizomucor miehei (Lipozyme IM-20) was used to catalyze the esterification reaction between isovaleric acid and ethanol to synthesize ethyl isovalerate in n-hexane. Response surface methodology based on five-level four-variable central composite rotatable design was employed to optimize four important reaction variables such as enzyme/substrate E/S ratio, substrate concentration, incubation time, and temperature affecting the synthesis of ethyl isovalerate. The optimum conditions predicted for achieving maximum ester yield (500 mM) are as follows: E/S ratio, 48.41 g/mol; substrate concentration, 1 M; reaction time, 60 h; temperature, 60 degrees C. The predicted value matched well with experimentally obtained value of 487 mM.
|
|
| Date |
2003
|
|
| Type |
Article
PeerReviewed |
|
| Format |
pdf
|
|
| Language |
en
|
|
| Identifier |
http://ir.cftri.com/2070/1/Applied%20Biochemistry%20and%20Microbiology%2C%20Vol.%2039%2C%20No.%203%2C%202003%2C%20pp.%20243%E2%80%93248.pdf
Chowdary, G. V. and Prapulla, S. G. (2003) Synthesis of ethyl isovalerate using Rhizomucor miehei lipase: optimization. Applied Biochemistry and Microbiology, 39 (3). pp. 278-83. |
|