CSIR Central

Reverse micellar extraction for downstream processing of proteins/enzymes.

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore


Field	Value

Relation	http://ir.cftri.com/2121/

Title	Reverse micellar extraction for downstream processing of proteins/enzymes.

Creator	Krishna, S. Hari Srinivas, N. D. Raghavarao, K. S. M. S. Karanth, N. G.

Subject	05 Enzymes 05 Processing and Engineering

Description	New developments in the area of downstream processing are, hopefully, to fulfill the promises of modern biotechnology. The traditional separation processes such as chromatography or electrophoresis can become prohibitively expensive unless the product is of high value. Hence, there is a need to develop efficient and cost-effective downstream processing methods. Reverse micellar extraction is one such potential and a promising liquid-liquid extraction technique, which has received immense attention for isolation and purification of proteins/enzymes in the recent times. This technique is easy to scale-up and offers continuous operation. This review, besides briefly considering important physico-chemical and biological aspects, highlights the engineering aspects including mass transfer, mathematical modeling, and technology development. It also discusses recent developments in reverse micellar extraction such as affinity based separations, enzymatic reactions in reverse micelles coupled with membrane processes, reverse micellar extraction in hollow fibers, etc. Special emphasis has been given to some recent applications of this technique.

Date	2002

Type	Article PeerReviewed

Format	pdf

Language	en

Identifier	http://ir.cftri.com/2121/1/Advances%20in%20biochemical%20engineeringbiotechnology%2C%20Volume-75%28%20%282002%29%20119-83.pdf Krishna, S. Hari and Srinivas, N. D. and Raghavarao, K. S. M. S. and Karanth, N. G. (2002) Reverse micellar extraction for downstream processing of proteins/enzymes. Advances in Biochemical Engineering/Biotechnology, 75. pp. 119-83. ISSN 0724-6145