Isolation and characterization of a sialo-glycopeptide from buffalo colostrum.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/2241/
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| Title |
Isolation and characterization of a sialo-glycopeptide from buffalo colostrum.
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| Creator |
Aparna, H. S.
Salimath, P. V. |
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| Subject |
25 Peptide Chemistry
03 Animal Husbandry |
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| Description |
A sialoglycopeptide was isolated from buffalo colostrum in pure form by chromatography on Sephadex G-25 and QAE-Sephadex A-25. This was found to be homogeneous by cellulose acetate membrane electrophoresis and reverse phase HPLC. It consisted of fucose, galactose, mannose, N-acetyl glucosamine and N-acetyl neuraminic acid in the ratio 1:2:3:4:1, and aspartic acid, serine, threonine, proline and glutamic acid were the major amino acids. Glycine was identified as the N-terminal amino acid residue. The structure elucidation of the carbohydrate moiety was carried out by methylation analysis, mass spectrometry. 1H-NMR spectroscopy and the probable structure was revealed to be that of a complex biantennary type.
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| Date |
1996
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/2241/1/Glycoconjugate%20Journal%20%281996%29%201363-67.pdf
Aparna, H. S. and Salimath, P. V. (1996) Isolation and characterization of a sialo-glycopeptide from buffalo colostrum. Glycoconjugate Journal, 13 (1). pp. 63-7. ISSN 0282-0080 |
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