Studies on the conformation of alpha-globulin from Sesamum indicum L. in cationic and nonionic detergents.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/5724/
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| Title |
Studies on the conformation of alpha-globulin from Sesamum indicum L. in cationic and nonionic detergents.
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| Creator |
Prakash, V.
Nandi, P. K. |
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| Subject |
03 Proteins
01 Oilseeds |
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| Description |
The circular dichroic spectra of a-globulin from Sesamum indicum L. was
recorded in the presence of cetyltrimethyl ammonium bromide, Triton X-100 and Brij-36T.
The protein in 0.2 Μ phosphate buffer pH 7.4 had about 25% β-structure and 5% α-helix, the
rest being aperiodic or irregular structure and a-helix, structure was increased by cationic
detergent cetyl trimethyl ammonium bromide. But, the increase in α-helix content was much
less than that induced by an anionic detergent, sodium dodecyl sulphate. In non-ionic
detergent like Brij-36T and Triton X-100, specific β-structures like II-β and I-β were formed
along with changes in α-helical and aperiodic structures. These results suggest that the
protein has a fairly labile quaternary structure.
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| Date |
1982
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/5724/1/Journal%20of%20Biosciences%2C%20Volume-4%283%20%281982%29%20331-345.pdf
Prakash, V. and Nandi, P. K. (1982) Studies on the conformation of alpha-globulin from Sesamum indicum L. in cationic and nonionic detergents. Journal of Biosciences, 4 (3). pp. 331-345. |
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