Micropropagation of Bixa orellana using phytohormones and triacontanol
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/9770/
BP-01-09 |
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| Title |
Micropropagation of Bixa orellana using phytohormones and triacontanol
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| Creator |
Parimalan, R.
Giridhar, P. Gururaj, H. B. Ravishankar, G. A. |
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| Subject |
06 Trees And Shrubs
05 Tissue Culture |
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| Description |
An efficient micropropagation protocol for annatto (Bixa orellana L.) was achieved using nodal shoot tip explants.
Shoot buds were obtained on the Murashige and Skoog (MS) medium supplemented with various concentrations and
combinations of indole-3-acetic acid (IAA), N6-benzyladenine (BA) and triacontanol (TRIA). Maximum of 213 shoot
buds along with 18 primary shoots were produced on MS medium containing 0.05 μM IAA, 8.87 μM BA, and 11.2 μM
TRIA. The primary shoots elongated best on MS medium containing 6.66 μM BA and 2.45 μM indole-3-butyric acid
(IBA). The regenerated shoots rooted best on MS medium supplemented with 4.9 μM IBA. The in vitro rooted plantlets
were hardened and establishment rate under field conditions was 70 to 80 %.
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/9770/1/BIOLOGIA_PLANTARUM_53_%282%29_347-350%2C_2009.pdf
Parimalan, R. and Giridhar, P. and Gururaj, H. B. and Ravishankar, G. A. (2009) Micropropagation of Bixa orellana using phytohormones and triacontanol. Biologia Plantarum, 53 (2). pp. 347-350. |
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