Insights on the age dependent neurodegeneration induced by Monocrotophos, (an organophosphorous insecticide) in Caenorhabditis elegans fed high glucose: Evidence in wild and transgenic strains.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13553/
https://doi.org/10.1016/j.cbpc.2018.05.002 |
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| Title |
Insights on the age dependent neurodegeneration induced by
Monocrotophos, (an organophosphorous insecticide) in Caenorhabditis
elegans fed high glucose: Evidence in wild and transgenic strains.
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| Creator |
Chinnu, Salim
Nidheesh, T. Muralidhara, M. Rajini, P. S. |
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| Subject |
26 Pesticide Chemistry
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| Description |
The higher susceptibility of high glucose fed C. elegans to Monocrotophos (MCP, an organophosphorus insecticide)
- induced dopaminergic (DA) neuronal degeneration was recently demonstrated. Employing this acute
exposure model, the impact of MCP on DA degeneration among worms of two age groups (8 and 13 d old) fed
control (CO) and high glucose (GF) diet with specific focus on phenotypic alterations, oxidative impairments and
associated molecular perturbations employing both wild (N2) and transgenic strains(BZ555 and NL5901) was
investigated. In general, 13 d worms exhibited higher susceptibility to MCP intoxication compared to 8 d old
worms. Further, MCP-exposure caused an enhanced degree of DA degeneration among glucose fed (GF) worms
as evidenced by lower chemotaxis index, reduced long-term memory and increased nonanone repulsion.
Biochemical analysis of 13 d GF worms also revealed a significant increase in ROS, protein carbonyls and reduced
ADP/ATP ratio. Interestingly, marked increase in degeneration of dopaminergic neurons and increased in
α-synuclein content was evident among 13 d GF worms exposed to MCP. Significant alterations in the mRNA
expression levels of daf-2, age-1, sir 2.1 and aak-2 among 13 d GF worms was evident. Collectively these findings
suggest that high intake of glucose diet aggravates MCP associated dopaminergic neuronal degeneration and the
impact of increasing age under such a condition. Moreover it provides an experimental paradigm to explore the
molecular targets and mechanism/s underlying the possible relationship between insecticide exposure-associated
dopaminergic degeneration in humans under hyperglycemic conditions.
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| Date |
2018
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13553/1/Comparative%20Biochemistry%20and%20Physiology%2C%20Part%20C%20211%20%282018%29%2015%E2%80%9324.pdf
Chinnu, Salim and Nidheesh, T. and Muralidhara, M. and Rajini, P. S. (2018) Insights on the age dependent neurodegeneration induced by Monocrotophos, (an organophosphorous insecticide) in Caenorhabditis elegans fed high glucose: Evidence in wild and transgenic strains. Comparative Biochemistry and Physiology, Part C, 211. pp. 15-24. |
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