The m6A methyltransferase Ime4 and mitochondrial functions in yeast.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13555/
https://dx.doi.org/10.1007/s00294-017-0758-8 |
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| Title |
The m6A
methyltransferase Ime4 and mitochondrial functions
in yeast.
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| Creator |
Pradeep Kumar, Yadav
Ram, Rajasekharan |
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| Subject |
03 Biochemistry & Molecular Biology
19 Yeast |
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| Description |
In eukaryotes, the precise transcriptional and
post-transcriptional regulations of gene expression are crucial
for the developmental processes. More than 100 types
of post-transcriptional RNA modifications have been identified
in eukaryotes. The deposition of N6-
methyladenosine
(
m6A) into mRNA is among the most common post-transcriptional
RNA modifications known in eukaryotes. It
has been reported that m6A
RNA modification can regulate
gene expression. The role of yeast m6A
methyltransferase
(Ime4) in meiosis and sporulation in diploid cells is
very well proven, but its physiological role in haploid cells
has remained unknown until recently. Previously, we have
shown that Ime4 epitranscriptionally regulates triacylglycerol
(TAG) metabolism and vacuolar morphology in haploid
cells. Mitochondrial dysfunction leads to TAG accumulation
as lipid droplets (LDs) in the cells; besides, LDs are
physically connected to the mitochondria. As of now there
are no reports on the role of Ime4 in mitochondrial biology.
Here we report the important role played by Ime4 in the
mitochondrial morphology and functions in Saccharomyces
cerevisiae. The confocal microscopic analysis showed that
IME4 gene deletion causes mitochondrial fragmentation;
besides, the ime4Δ cells showed a significant decrease in
cytochrome c oxidase and citrate synthase activities compared
to the wild-type cells. IME4 gene deletion causes mitochondrial dysfunction, and it will be interesting to find
out the target genes of Ime4 related to the mitochondrial
biology. The determination of the role of Ime4 and its targets
in mitochondrial biology could probably help in formulating
potential cures for the mitochondria-linked rare genetic
disorders.
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| Date |
2018
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13555/1/Curr%20Genet%20%282018%29%2064353%E2%80%93357.pdf
Pradeep Kumar, Yadav and Ram, Rajasekharan (2018) The m6A methyltransferase Ime4 and mitochondrial functions in yeast. Current Genetics, 64. pp. 353-357. |
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