Effects of methyl jasmonate and carotenogenic inhibitors on gene expression and carotenoid accumulation in coriander (Coriandrum sativum L.) foliage.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13563/
https://doi.org/10.1016/j.foodres.2018.04.040 |
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| Title |
Effects of methyl jasmonate and carotenogenic inhibitors on gene expression
and carotenoid accumulation in coriander (Coriandrum sativum L.) foliage.
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| Creator |
Divya, P.
Bijesh, Puthusseri Savanur, M. A. Veeresh, Lokesh Bhagyalakshmi, N. |
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| Subject |
14 Carotenoid Chemistry
30 Spices/Condiments |
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| Description |
Coriander (Coriandrum sativum L.), a commonly used annual herb that accumulates carotenoids upon methyl
jasmonate (MeJA) treatment, provides an excellent model to investigate carotenogenesis and gene regulation. To
explore key mechanisms involved in enhancing carotenoids, transcriptional expression profile of ten carotenogenic
genes in the presence of MeJA and various gene specific inhibitors were investigated. Foliar application of
MeJA (10 μM) increased expression levels of CsPDS (phytoene desaturase), CsZDS (ς-carotene desaturase),
CsCHYE (carotene ε - hydroxylase) and CsLCYE (lycopene β-cyclase) genes, and their transcript levels were
strongly associated with carotenoid content, where, three days after treatment, 3.9 & 6.1 fold increase was
observed for β-carotene and lutein respectively. The regulatory effect of key genes, CsPDS, CsZDS, CsLCYE and
LCYB were further confirmed by using gene-specific inhibitors fosmidomycin, norflurazon and amitrol.
Norflurazon- the phytoene desaturation inhibitor leads to a decrease in β-carotene and lutein content correlated
with CsPDS, CsZDS gene induction. Our results clearly demonstrate that MeJA induced-signalling network
evokes carotenogenic genes, leading to the accumulation of carotenoids. This knowledge may help to develop
precise strategies for remodelling carotenoid pathway so that desired levels of a particular carotenoid in leafy
vegetables is achievable.
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| Date |
2018
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13563/1/Food%20Research%20International%20111%20%282018%29%2011%E2%80%9319.pdf
Divya, P. and Bijesh, Puthusseri and Savanur, M. A. and Veeresh, Lokesh and Bhagyalakshmi, N. (2018) Effects of methyl jasmonate and carotenogenic inhibitors on gene expression and carotenoid accumulation in coriander (Coriandrum sativum L.) foliage. Food Research International, 111. pp. 11-19. |
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