Antagonistic effect of Saccharomyces cerevisiae KTP and Issatchenkia occidentalis ApC on hyphal development and adhesion of Candida albicans.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13585/
https://dx.doi.org/10.1093/mmy/myx156 |
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| Title |
Antagonistic effect of Saccharomyces cerevisiae
KTP and Issatchenkia occidentalis ApC on
hyphal development and adhesion of Candida
albicans.
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| Creator |
Lohith, K.
Anu Appaiah, K. A. |
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| Subject |
04 Yeast
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| Description |
The morphological transition from yeast to a hyphal form, as well as the adhesion
capability to the gastrointestinal tract, are implicated virulent determinant in
Candida albicans and could be potential targets for prevention of the opportunistic
pathogen. Based on this rationale, two yeast strains Saccharomyces cerevisiae KTP
and Issatchenkia occidentalis ApC along with reference strain Saccharomyces boulardii
NCDC 363 were screened for the probiotic potential. Characters like pH, temperature,
bile, simulated gastrointestinal juice tolerance tests, and Caco-2 cell line adhesion
assay were determined in the present study. Further, the evaluation of its impact
on C. albicans morphological transition and adhesion was assessed using microtitre
germ tube test. In terms of probiotic characteristics, both the strains were tolerant to pH
2.5 and the presence of bile (0.3 to 0.6%) with an optimum growth temperature of 37◦C.
The strain KTP was also resistant to simulated gastric and intestinal juices as compared
to control (13% and 41%, respectively) and NCDC 363 (55% and 35%, respectively). In
contrast, both the yeasts had reduced adhesiveness to Caco-2 monolayer. Candida virulence
in in vitro systems indicated that treatment of live probiotic yeast cells (108 ml)
effectively reduced the filamentation and adhesion of C. albicans. The S. cerevisiae KTP
had a profound effect on the hyphal development and adhesion when compared to the
ApC and NCDC 363. The strain significantly reduced (P < .05) the hyphal growth in cocultivated
(93% and 94%, respectively) and pre-existing hyphae (54% and 68%) of strains
C. albicans 183 and 1151. Isolates KTP and ApC also reduced the adhesion (≈ 22% and
41%, respectively) and transition of blastoconidia at two hours of incubation in abiotic
surface. This study provides knowledge on the effect of potential probiotic yeasts such as Saccharomyces and non- Saccharomyces strains against virulence characteristic of Candida
albicans.
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| Date |
2017
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13585/1/Medical%20Mycology%2C%202017%2C%200%2C%201%E2%80%9310.pdf
Lohith, K. and Anu Appaiah, K. A. (2017) Antagonistic effect of Saccharomyces cerevisiae KTP and Issatchenkia occidentalis ApC on hyphal development and adhesion of Candida albicans. Medical Mycology. pp. 1-10. |
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