Expression profiling of the Dolichos lablab lectin during germination and development of the seed.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13590/
https://dx.doi.org/10.1016/j.plaphy.2017.12.040 |
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| Title |
Expression profiling of the Dolichos lablab lectin during germination and development
of the seed.
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| Creator |
Yashavanth, L. Vishweshwaraiah
Balaji, Prakash Lalitha, R. Gowda |
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| Subject |
03 Proteins
22 Legumes-Pulses |
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| Description |
The temporal expression of the field bean (Dolichos lablab) galactose specific lectin, DLL-II, during
germination, post-germination and seed development was evaluated using Native-PAGE followed by
activity staining, immunodetection and quantitative Real Time PCR (qPCR). A rapid and steep
decline in the polyphenol oxidase (PPO) and hemagglutinating activity during the initial stages of
germination, which did not correlate with the slow decline in total protein was observed. During post
germination period, PPO and hemagglutination activities were negligible, whereas a rapid resorption
of the protein was evident. These results suggest that DLL-II is not a storage protein. The presence of
mRNA in the quiescent seed and initial stages of germination are indicative of a very stable mRNA.
DLL-II was expressed in high copies during seed development and increased dramatically between
10 - 20 days after flowering (DAF), suggesting a switch over stage in DLL-II expression. Transcript
levels reached a maximum at the mature stage of seed development. Among the non-seed tissues
examined, root showed the highest level. The high affinity binding to kinetin and indole acetic acid,
the key hormones that regulate root development and its vascular differentiation add a new dimension
to the physiological role of DLL-II in the seed. This finding, coupled with the PPO and
hemagglutinating activity makes DLL-II, a truly a multifunctional protein.
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| Date |
2018
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13590/1/Plant%20Physiology%20et%20Biochemistry%202018.pdf
Yashavanth, L. Vishweshwaraiah and Balaji, Prakash and Lalitha, R. Gowda (2018) Expression profiling of the Dolichos lablab lectin during germination and development of the seed. Plant Physiology and Biochemistry, 124. pp. 10-19. |
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