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Optimization of polyhydroxybutyrate production by β-carotene producing strain of Micrococcus sp.

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore

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Relation http://ir.cftri.com/8888/
JFST-05-08
 
Title Optimization of polyhydroxybutyrate production by β-carotene producing strain of Micrococcus sp.
 
Creator Vijayendra, S. V. N.
Veeramani, S.
Shamala, T.R
 
Subject 04 Microbiology
 
Description Optimization of the polyhydroxybutyrate (PHB) production by a pigmented Micrococcus sp. was done by using glucose, sucrose, lactose and fructose as carbon sources, initial pH between 6.0 and 8.0, fermentation temperature of 30 and 37°C, inoculum of 5, 10 and 15% v/v and by submerged/aerobic fermentation. Maximum production of polyhydroxyalkanoates (PHA) was at 37°C in sucrose containing pH 6.0 under aerobic conditions. Overall, the PHB production ranged between 35 and 40% of the dry cell weight. In GC analysis, PHA and PHB produced the same peak. The UV-visible spectrophotometer and HPLC analysis of the pigment indicated presence of (β-carotenoids with absorption maxima at 454 and 348 nm.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/8888/1/JFST_45%286%29_2008_506-509.pdf
Vijayendra, S. V. N. and Veeramani, S. and Shamala, T.R (2008) Optimization of polyhydroxybutyrate production by β-carotene producing strain of Micrococcus sp. Journal of Food Science and Technology, 45 (6). pp. 506-509.