Probiotic attributes, antioxidant, anti-inflammatory and neuromodulatory effects of Enterococcus faecium CFR 3003: in vitro and in vivo evidence.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13810/
https://dx.doi.org/10.1099/jmm.0.000184 |
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| Title |
Probiotic attributes, antioxidant, anti-inflammatory
and neuromodulatory effects of Enterococcus
faecium CFR 3003: in vitro and in vivo evidence.
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| Creator |
Divyashri, G.
Krishna, G. Dr., Muralidhara Prapulla, S. G. |
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| Subject |
02 Bacteriology
32 Antioxidants |
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| Description |
Accumulating evidence suggests that probiotic bacteria play a vital role in modulating various
aspects integral to the health and well-being of humans. In the present study, probiotic attributes
and the antioxidant, anti-inflammatory and neuromodulatory potential of Enterococcus faecium
CFR 3003 were investigated by employing suitable model systems. E. faecium exhibited robust
resistance to gastrointestinal stress conditions as it could withstand acid stress at pH 1.5, 2 and
3. The bacterium also survived at a bile salt concentration of 0.45 %, and better tolerance was
observed towards pepsin and trypsin. E. faecium produced lactic acid as a major metabolic
product, followed by butyric acid. Lyophilized cell-free supernatant (LCS) of E. faecium exhibited
significant antioxidant capacity evaluated against 1,1-diphenyl-2-picryl-hydrazyl, ascorbate autooxidation,
oxygen radical absorbance and reducing power. Interestingly, E. faecium, Lactobacillus
rhamnosus GG MTCC 1408 and LCS showed a significant anti-inflammatory effect by negatively
modulating TNF-a production and upregulating IL-10 levels in LPS-stimulated macrophage cell
lines. In an in vivo mice model, the propensity of probiotic supplements to modulate endogenous
oxidative markers and redox status in brain regions was assessed. Young mice provided with oral
supplements (daily for 28 days) of E. faecium and L. rhamnosus exhibited diminished oxidative
markers in the brain and enhanced activities of antioxidant enzymes with a concomitant increase in
c-aminobutyric acid and dopamine levels. Collectively, our findings clearly suggest the propensity
of these bacteria to protect against tissue damage mediated through free radicals and
inflammatory cytokines. Although the underlying molecular mechanisms need further studies, it is
tempting to speculate that probiotics confer a neuroprotective advantage in vivo against oxidative
damage-mediated neurodegenerative conditions.
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| Date |
2015
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13810/1/Journal%20of%20Medical%20Microbiology%20%282015%29%2C%2064%2C%201527%E2%80%931540.pdf
Divyashri, G. and Krishna, G. and Dr., Muralidhara and Prapulla, S. G. (2015) Probiotic attributes, antioxidant, anti-inflammatory and neuromodulatory effects of Enterococcus faecium CFR 3003: in vitro and in vivo evidence. Journal of Medical Microbiology, 64. pp. 1527-1540. |
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