Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/13817/
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| Title |
Quantification of Neurotransmitters from Intact
and Regenerating Planarians Using UHPLC-MS/SRM Method.
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| Creator |
Kannan, Rangiah
Palakodeti, Dasaradhi |
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| Subject |
06 Chromatography
Fish |
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| Description |
Freshwater planarian species S. mediterranea is an emerging stem cell model because of its capability of
regenerating large portions of missing body parts. It is one of the best model systems available to address
the basic biological mechanisms in the regeneration processes. Absolute quantification of metabolites from
planarians is imperative to understand their role in the regeneration processes. Here we describe a stable
isotope dilution ultrahigh performance liquid chromatography/mass spectrometry/selected reaction
monitoring (UHPLC-MS/SRM) assay for a sensitive and quantitative assessment of neurotransmitters
(NTs) in planaria. We used this method for the simultaneous quantification of 16 NTs from both intact
and regenerating planarians.
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| Publisher |
Springer
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| Date |
2018
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| Type |
Book Section
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13817/1/Methods%20in%20Molecular%20Biology%2C%20vol.%201774.pdf
Kannan, Rangiah and Palakodeti, Dasaradhi (2018) Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method. In: Planarian Regeneration: Methods and Protocols, Methods in Molecular Biology. Springer, pp. 555-570. |
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