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Arabidopsis serine/threonine/ tyrosine protein kinase phosphorylates oil body proteins that regulate oil content in the seeds.

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore

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Relation http://ir.cftri.com/13831/
https://dx.doi.org/10.1038/s41598-018-19311-3
 
Title Arabidopsis serine/threonine/ tyrosine protein kinase phosphorylates oil body proteins that regulate oil content in the seeds.
 
Creator Iyappan, R.
Anitha, Vijayakumar
Ramya, V.
Ram, Rajasekharan
 
Subject 10 Plants
03 Proteins
19 Lipids-oils/fats
 
Description Protein phosphorylation is an important post-translational modification that can regulate the protein function. The current knowledge on the phosphorylation status of plant oil body (OB) proteins is inadequate. This present study identifies the distinct physiological substrates of Arabidopsis serine/ threonine/tyrosine protein kinase (STYK) and its role in seed oil accumulation; the role of Arabidopsis OLE1, a major seed OB protein has also been elucidated. In vitro kinase assay followed by mass spectrometry identifies residue that are phosphorylated by STYK. Further, co-expression of OLE1 and STYK in yeast cells increases the cellular lipid levels and reduces the total lipid when OLE1 was replaced with OLE1T166A. Moreover, in vivo experiments with OB isolated from wild-type and styk knock-out lines show the ability of STYK to phosphorylate distinct OB proteins. OLE1T166A mutant and Arabidopsis styk mutant demonstrate the significant reduction of its substrate phosphorylation. styk mutant line significantly reduces the amount of total seed oil as compared to wild-type seeds. Together, our results provide the evidences that Arabidopsis At2G24360 (STYK) is phosphorylating oil body proteins and the phosphorylation regulates the oil content in Arabidopsis seeds.
 
Date 2018
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13831/1/SCientifiC%20REPOrTS%20%20%282018%29%2081154.pdf
Iyappan, R. and Anitha, Vijayakumar and Ramya, V. and Ram, Rajasekharan (2018) Arabidopsis serine/threonine/ tyrosine protein kinase phosphorylates oil body proteins that regulate oil content in the seeds. Scientific Reports, 8 (1154). pp. 1-14.