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A simple reversed-phase HPLC method for the estimation of dimethylarginines and homocysteine in plasma and serum.

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore


Field	Value

Relation	http://ir.cftri.com/13834/ https://dx.doi.org/10.1002/sscp.201800038

Title	A simple reversed-phase HPLC method for the estimation of dimethylarginines and homocysteine in plasma and serum.

Creator	Venkatesh, R. Sridevi Annapurna, Singh

Subject	06 Chromatography

Description	Elevated concentrations of asymmetric dimethylarginine, symmetric dimethylarginine and homocysteine are considered as common risk factors for cardiovascular diseases. A simple reversed-phase high-performance liquid chromatography method has been developed to simultaneously detect these biomarkers in plasma and serum. Straightforward sample preparation involves direct derivatization of deproteinized serum/plasma with phenyl isothiocyanate. Dimethylarginines and homocysteine are resolved using a 250 × 4.6 mm I.D, C18 column and a linear gradient of sodium acetate buffer/acetonitrile. Calibration curves of peak area verses concentration are linear with correlation coefficients near unity. The intraday and interday precisions measured by coefficient of variation of peak areas were 1.34–9.7, 2.7–6.7 and 0.3– 7.1 for asymmetric dimethylarginine, symmetric dimethylarginine and homocysteine, respectively. The recoveries of asymmetric dimethylarginine, symmetric dimethylarginine and homocysteine are above 86%.Minimal sample preparation, good recoveries and fast run time makes this method suitable for the analysis of plasma and serum.

Date	2018

Type	Article PeerReviewed

Format	pdf

Language	en

Identifier	http://ir.cftri.com/13834/1/Sep%20Sci%20plus%202018%3B1404%E2%80%93410.pdf Venkatesh, R. and Sridevi Annapurna, Singh (2018) A simple reversed-phase HPLC method for the estimation of dimethylarginines and homocysteine in plasma and serum. SSC Plus, 1 (6). pp. 404-410.