In vitro adhesion and anti-inflammatory properties of native Lactobacillus fermentum and Lactobacillus delbrueckii spp.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/13836/
https://dx.doi.org/10.1111/jam.13757 |
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| Title |
In vitro adhesion and anti-inflammatory properties of
native Lactobacillus fermentum and Lactobacillus
delbrueckii spp.
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| Creator |
Archer, A. C.
Kurrey, N. K. Prakash, M. Halami |
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| Subject |
02 Bacteriology
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| Description |
Aims: This study aimed at characterizing the adhesion and immunestimulatory
properties of native probiotic Lactobacillus fermentum (MCC 2759
and MCC 2760) and Lactobacillus delbrueckii MCC 2775.
Methods and Results: Adhesion of the strains was assessed in Caco-2 and HT-
29 cell lines. Expression of adhesion and immune markers were evaluated in
Caco-2 cells by real-time qPCR. The cultures displayed >80% of adhesion to
both cell lines and also induced the expression of mucin-binding protein
(mub) gene in the presence of mucin, bile and pancreatin. Adhesion was
mediated by carbohydrate and proteinaceous factors. The cultures stimulated
the expression of inflammatory cytokines in Caco-2 cells. However, proinflammatory
genes were down-regulated upon challenge with
lipopolysaccharide and IL-10 was up-regulated by the cultures. Cell wall extract
of L. fermentum MCC 2760 induced the expression of IL-6 by 5�47-fold,
whereas crude culture filtrate enhanced the expression of IL-10 by 14�87-fold
compared to LPS control.
Conclusions: The bacterial cultures exhibited strong adhesion and antiinflammatory
properties.
Significance and Impact of the Study: This is the first report to reveal the role
of adhesion markers of L. fermentum and L. delbrueckii by qPCR. The strainspecific
anti-inflammatory property of native cultures may be useful to alleviate
inflammatory conditions and develop a target-based probiotic.
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| Date |
2018
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/13836/1/Journal%20of%20Applied%20Microbiology%20125%2C%20243--256.pdf
Archer, A. C. and Kurrey, N. K. and Prakash, M. Halami (2018) In vitro adhesion and anti-inflammatory properties of native Lactobacillus fermentum and Lactobacillus delbrueckii spp. Journal of Applied Microbiology, 125. pp. 243-256. ISSN 1364-5072 |
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