Yeast MRX deletions have short chronological life span and more triacylglycerols.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/12719/
http://dx.doi.org/10.1093/femsyr/fov109 |
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| Title |
Yeast MRX deletions have short chronological life span
and more triacylglycerols.
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| Creator |
Kanaga Vijayan, D.
Ram, Rajasekharan Malathi, Srinivasan |
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| Subject |
22 Lipid Chemistry
19 Yeast |
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| Description |
Saccharomyces cerevisiae is an excellent model organism for lipid research. Here, we have used yeast haploid RAdiation
Damage (RAD) deletion strains to study life span and lipid storage patterns. RAD genes are mainly involved in DNA repair
mechanism and hence, their deletions have resulted in shorter life span. Viable RAD mutants were screened for non-polar
lipid content, and some of the mutants showed significantly high amounts of triacylglycerol (TAG) and steryl ester, besides
short chronological life span. Among these, RAD50, MRE11 and XRS2 form a complex, MRX that is involved in homologous
recombination that showed an increase in the amount of TAG. Microarray data of single MRX deletions revealed that
besides DNA damage signature genes, lipid metabolism genes are also differentially expressed. Lipid biosynthetic genes
(LPP1, SLC1) were upregulated and lipid hydrolytic gene (TGL3) was downregulated. We observed that rad50�, mre11�, xrs2�
and mrx� strains have high number of lipid droplets (LDs) with fragmented mitochondria. These mutants have a short
chronological life span compared to wild type. Aged wild-type cells also accumulated TAG with LDs of ∼2.0 μm in diameter.
These results suggest that TAG accumulation and big size LDs could be possible markers for premature or normal aging.
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/12719/1/FEMS%20Yeast%20Research%2C%2016%2C%202016.pdf
Kanaga Vijayan, D. and Ram, Rajasekharan and Malathi, Srinivasan (2016) Yeast MRX deletions have short chronological life span and more triacylglycerols. FEMS Yeast Research, 16 (1). pp. 1-14. |
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