Lipase-Catalyzed Synthesis of Isoamyl Butyrate: Optimization by Response Surface Methodology
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/9139/
JAOCS-13-99 |
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| Title |
Lipase-Catalyzed Synthesis of Isoamyl Butyrate:
Optimization by Response Surface Methodology
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| Creator |
Hari Krishna, S.
Manohar, B. Divakar, S. Karanth, N. G. |
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| Subject |
05 Enzymes
05 Processing and Engineering |
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| Description |
Immobilized lipase from Mucor miehei (Lipozyme
IM-20) was employed in the esterification of butyric acid
and isoamyl alcohol to synthesize isoamyl butyrate in n-hexane.
Response surface methodology based on five-level, five-variable
central composite rotatable design was used to evaluate
the effects of important variables—enzyme/substrate (E/S) ratio
(5–25 g/mol), acid concentration (0.2–1.0 M), alcohol concentration
(0.25–1.25 M), incubation period (12–60 h), and temperature
(30–50°C)—on esterification yield of isoamyl butyrate.
In the range of parameters studied, the extent of esterification
decreased with temperature, lower E/S ratios, and incubation
periods. Excess acid and alcohol concentrations (i.e., acid/alcohol
> 1.4 or alcohol/acid > 1.4) were found to decrease yield
probably owing to inhibition of the enzyme by acid or alcohol,
the former being more severe. The optimal conditions achieved
are as follows: E/S ratio, 17 g/mol; acid concentration, 1.0 M;
incubation period, 60 h; alcohol concentration, 1.25 M; and
temperature, 30°C. With these conditions, the predicted value
was 1.0 M ester, and the actual experimental value was 0.98 M.drawback.
Considering the high demand and benefits, an optimized
process with high yields for the economic enzymatic
synthesis of isoamyl butyrate is important.
The goal of the present investigation was to optimize the
process for enzymatic synthesis of isoamyl butyrate using response
surface methodology employing a five-level, five-variable
central composite rotatable design (CCRD). The variables
affecting the esterification that were considered were enzyme/
substrate (E/S) ratio, acid concentration, incubation period,
alcohol concentration, and reaction temperature.
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| Date |
1999
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/9139/1/JAOCS%2C_Vol._76%2C_no._12_%281999%29_1483.pdf
Hari Krishna, S. and Manohar, B. and Divakar, S. and Karanth, N. G. (1999) Lipase-Catalyzed Synthesis of Isoamyl Butyrate: Optimization by Response Surface Methodology. Journal of the American Oil Chemists' Society, 76 (12). pp. 1483-1488. |
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