Mixed reverse micellar systems for extraction and purification of beta-glucosidase
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/10131/
SPT-01-10 |
|
| Title |
Mixed reverse micellar systems for extraction and purification of beta-glucosidase
|
|
| Creator |
Hemavathi, A. B.
Umesh Hebbar, H. Raghavarao, K. S. M. S. |
|
| Subject |
04 Microbiology
01 Analysis |
|
| Description |
Mixed reverse micellar (MRM) systems of sodium bis(2-ethylhexyl)sulfosuccinate (AOT) and nonionic
surfactants (Tween 20, Tween 80, Tween 85 and Triton X-100) in iso-octane were used for the extraction
and primary purification of �-glucosidase (EC 3.2.1.21) from the aqueous extract of barley (Hordeum
vulgare) for the first time. Studies are carried out with both phase transfer as well as injection mode
of reverse micellar extraction, among which, injection mode is observed to be more suitable for �-
glucosidase extraction. The process parameters such as concentration of surfactants and their molar
ratio, type of solvent, volume of sample injected and its protein concentration, pH and ionic strength of
the initial aqueous phase for forward extraction, buffer pH, concentration of hexanol and salt during back
extraction are varied to maximize the extraction efficiency. The molar ratio of AOT:nonionic above 4.5:1.0
only resulted in clear phase formation and below this ratio precipitation was observed at the interface
during forward extraction. Mixed reverse micelles found to solubilize almost three times more volume of
water and protein than AOT alone under otherwise similar conditions. Activity recovery was found to be
in the order of AOT/Tween 20 > AOT/Tween 80 > AOT/Triton X-100 > AOT/Tween 85 > AOT. MRM system
of 150mM AOT/15mM Tween 20 has resulted in maximum activity recovery of 95.18% and degree of
purification of 4.8-fold.
|
|
| Date |
2010
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Language |
en
|
|
| Identifier |
http://ir.cftri.com/10131/1/Separation_and_Purification_Technology%2C_Volume_71%2C_Issue_2%2C_18_February_2010%2C_Pages_263-268.pdf
Hemavathi, A. B. and Umesh Hebbar, H. and Raghavarao, K. S. M. S. (2010) Mixed reverse micellar systems for extraction and purification of beta-glucosidase. Separation and Purification Technology, 71. pp. 263-268. |
|