Development of cellulase-free xylanase-producing anaerobic consortia for the use of lignocellulosic wastes.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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Relation |
http://ir.cftri.com/7860/
EMT-02-96 |
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Title |
Development of cellulase-free xylanase-producing anaerobic consortia for the use of lignocellulosic wastes.
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Creator |
Rani, S.
Nand, K. |
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Subject |
04 Microbiology
02 Bacteriology 16 Enzyme Chemistry |
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Description |
Consortia of anaerobic bacteria producing xylan degrading enzymes were developed from samples collected from various ecosystems. Consortia developed included a thermophilic consortium (KK-10) producing cellulase-free xylanase (410 nkat ml-1) at 65-85C, pH 7-9, and mesophilic consortia (PC-4, CC-2 and BD-2) exhibiting xylanase activities of 580, 275 and 300 nkat ml-1, respectively, but also producing cellulases in the culture medium. Temp. and pH influenced the yield and pattern of xylanase and cellulase production markedly, with the optimum at 72 h of growth and pH 6.7. Max. xylanase activity was recorded for all consortia at 37C except PC-4 and KK-10 consortia, which showed their highest activity at 55 and 65C, respectively. BD-2 consortium when cultivated at 55C exhibited a several-fold increase in beta-glucosidase activity as compared with other consortia. It also had a broad pH range of 5-9 for growth and xylanase production. A pH of 6-7 was optimum for other consortia. Max. xylanase activity of 592 nkat ml-1 was detected with PC-4 consortium. The xylanase of KK-10 could find industrial applications at higher temp., whereas neutral xylanases may be used in the food-processing industries. [From En summ.]
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Date |
1996
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Identifier |
http://ir.cftri.com/7860/1/Enzyme_%26_Microbial_Technology_18%281%29_1996_23-28.pdf
Rani, S. and Nand, K. (1996) Development of cellulase-free xylanase-producing anaerobic consortia for the use of lignocellulosic wastes. Enzyme and Microbial Technology, 18 (1). 23-28, 24 ref.. |
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