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Esterification and Interesterification Reactions of Lipases from Rice Bran and Papain in Oleic Acid-Glycerol/n-Butanol and two Vegetable Oil Blends

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore

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Relation http://ir.cftri.com/9649/
JLST-01-09
 
Title Esterification and Interesterification Reactions of Lipases from Rice Bran and Papain in Oleic Acid-Glycerol/n-Butanol and two Vegetable Oil Blends
 
Creator Prasanth Kumar, P. K.
Gopala Krishna, A. G.
 
Subject 05 Enzymes
19 Lipids-oils/fats
 
Description The ability of crude rice bran lipase and commercial papain lipase was evaluated for esterification of oleic acid with glycerol and n-butanol and interesterification of palm oil and rice bran oil blend. For esterification of oleic acid with glycerol / nbutanol, the reaction proceeded upto 1.9%, 3.0% (37UC), 2.5%, 8.6% (50UC) at atmospheric pressure and 1.5%, 8.3% (37UC) 7.3%, 6.4% (50UC) under reduced pressure for rice bran and papain lipases respectively. During the interesterification reaction, concentration of LLL decreased by 24.5, 38.5 and 6.3%; PPP decreased by 73.9, 68.1 and 14%; LLP / LPL decreased by 11.6, 28.2 and 15.2% with an increase in the concentration of LOL (134.4, 78.7 and 120.3%), POO (9.1, 10.5 and 2.7%) and PPO (4.9, 10.1, 2.3%) for the three studied enzymes viz., lipozyme IM-60 (as a control), rice bran and papain lipases respectively. The physical state of the blend after treatment with lipozyme IM-60, crude rice bran and papain lipases has changed from semisolid to completely liquid state even at 5-10°C while the physical blend was a semisolid at RT / 25°C / 5-10°C. It is concluded from the study that these enzymes can be used for carrying out esterification and interesterification reactions which is being reported for the first time.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/9649/1/JLST_41%283%29.pdf
Prasanth Kumar, P. K. and Gopala Krishna, A. G. (2009) Esterification and Interesterification Reactions of Lipases from Rice Bran and Papain in Oleic Acid-Glycerol/n-Butanol and two Vegetable Oil Blends. Journal of Lipid Science and Technology, 41 (3). pp. 88-94.