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Serological evidence for the occurrence of races in Sclerospora graminicola and identification of a race-specific surface protein involved in host recognition

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore

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CJB-02-93
 
Title Serological evidence for the occurrence of races in Sclerospora graminicola and identification of a race-specific surface protein involved in host recognition
 
Creator Vasantha, U. Kumar
Shishupala, S.
Shetty, H. S.
 
Subject 04 Microbiology
 
Description A protein specific to a virulent pathotype of the downy mildew pathogen Sclerospora grarninicola was identified on the Western transfers using the cross-adsorbed antibody. This protein was approximately 90 kDa and without subunits. Serological agglutination studies localized the protein on the cell wall surfaces. ~ n z ~ mlineke d immunosorbent assay and dot blotting using the specific antibodies identified distinct races of the pathogen. Immunofluorescence studies and enzyme linked immunosorbent assay using suspension cultures of host cells showed that the protein bound to the cells of susceptible cultivar but not to those of resistant cultivar. These results suggested that this protein may be involved in host recognition.
 
Date 1993
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://ir.cftri.com/9310/1/CAN_J_Bot_71_1993_1467.pdf
Vasantha, U. Kumar and Shishupala, S. and Shetty, H. S. (1993) Serological evidence for the occurrence of races in Sclerospora graminicola and identification of a race-specific surface protein involved in host recognition. Canadian Journal of Botany, 71. pp. 1467-1471.