Bioavailability and metabolism of fucoxanthin in rats: structural characterization of metabolites by LC-MS (APCI)
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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| Relation |
http://ir.cftri.com/9371/
MCB-02-10 |
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| Title |
Bioavailability and metabolism of fucoxanthin in rats: structural
characterization of metabolites by LC-MS (APCI)
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| Creator |
Ravi Kumar, Sangeetha
Narayan, Bhaskar Soundar, Divakar Vallikannan, Baskaran |
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| Subject |
01 Algae
04 Vitamins |
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| Description |
This study reports bioavailability and metabolism
of fucoxanthin (FUCO) from brown algae Padina
tetrastromatica in rats. Rats were divided into two
groups (n = 25/group). Group one was fed basal diet
(control) while the group two received retinol deficient diet
(RD group) for 8 weeks. After confirmed RD in blood
(0.53 lmol/l), rats were further sub-grouped (n = 5/sub
group), intubated a dose of FUCO (0.83 lmol) and killed
after 0, 2, 4, 6 and 8 h. The plasma levels (area under
curve/8 h) of FUCO (fucoxanthinol (FUOH) ? amarouciaxanthin
(AAx)) was 2.93 (RD group) and 2.74 pmol/dl
(control), respectively. No newly formed retinol was
detected in RD rats intubated with FUCO. Besides FUOH
(m/z 617 (M?H)?) and AAx (m/z 617 (M?H-)?), other
deacetylated, hydrolyzed and demethylated metabolites of
bearing molecular mass at m/z 600.6 (FUOH–H2O), m/z
597 (AAx–H2O), m/z 579 (AAx–2H2O?1), m/z 551 (AAx–
2H2O–2CH3?2) and m/z 523 (AAx–2H2O–4CH3?4) were
also detected in plasma and liver by LC-MS (APCI).
Although biological functions of FUCO metabolites need
thorough investigation, this is the first detailed report on
FUCO metabolites in rats.
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/9371/1/Mol_Cell_Biochem_%282010%29_333_299%E2%80%93310.pdf
Ravi Kumar, Sangeetha and Narayan, Bhaskar and Soundar, Divakar and Vallikannan, Baskaran (2010) Bioavailability and metabolism of fucoxanthin in rats: structural characterization of metabolites by LC-MS (APCI). Molecular and Cellular Biochemistry, 333. pp. 299-310. |
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