Purification and Partial Characterization of Intracellular Fructosyltransferase from a Novel Strain of Aureobasidium pullulans
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/9743/
TJB-02-07 |
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| Title |
Purification and Partial Characterization of
Intracellular Fructosyltransferase from a Novel Strain of
Aureobasidium pullulans
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| Creator |
Agbaje, Lateef
Julius Kola, Oloke Prapulla, S. G. |
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| Subject |
05 Enzymes
04 Fungi |
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| Description |
The intracellular fructosyltransferase (FTase) of a novel strain of Aureobasidium pullulans (CFR 77) capable of producing
59% of fructooligosaccharides (FOS) within 9 h of reaction time was obtained by wet-milling, and then purified and characterized.
The purified FTase revealed 2 bands of 147 and 170 KD; its activity was optimum at an approximate pH of 5.0 and temperature
of 55 °C. The specific activity of the final purified material was 42, representing a purification factor of 79.44 and yield of 43%.
The enzyme is very stable, retaining more than 80% of its original activity at the optimum reaction conditions after 12 h. Using the
crude intracellular FTase, 59% of FOS was produced within 9 h of reaction time, which is a considerable reduction in the reaction
time of 12-25 h that has been reported in the literature. The purified FTase yielded 59% of FOS within 3 h of reaction time.
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/9743/1/Turk_J_Biol_31%282007%29_147-.pdf
Agbaje, Lateef and Julius Kola, Oloke and Prapulla, S. G. (2007) Purification and Partial Characterization of Intracellular Fructosyltransferase from a Novel Strain of Aureobasidium pullulans. Turkish Journal of Biology, 31. pp. 147-154. |
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