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Comparison of activity and conformational changes of ficin during denaturation by urea and guanidine hydrochloride

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore


Field	Value

Relation	http://ir.cftri.com/10127/ PB-13-11

Title	Comparison of activity and conformational changes of ficin during denaturation by urea and guanidine hydrochloride

Creator	Devaraj, K. B. Ramesh Kumar, Parigi Prakash, V.

Subject	29 Protein Chemistry 06 Trees And Shrubs 05 Enzymes

Description	The activity and conformational changes of ficin (EC 3.4.22.3), a cysteine protease from Ficus carica have been investigated during the denaturation by urea and guanidine hydrochloride (GuHCl). The denaturation of ficin was followed by activity measurements, fluorescence and circular dichroism (CD) spectroscopic studies. The enzyme activity decreased significantly at low concentration of both urea and GuHCl before unfolding of the enzyme molecule. The enzyme molecule was resistant for unfolding by urea under neutral conditions even at higher concentrations. However, the protein is susceptible to unfolding by urea at lower pH and transition follows a cooperative two-state rule with increasing concentration of urea. On the other hand, ficin molecule loses its complete structure in presence of 4M GuHCl under neutral conditions. The GuHCl-induced unfolding occurs in a simple two-state cooperative process. These results indicate the differential structural stability and fragility of active site of the enzyme towards denaturation by urea and GuHCl.

Date	2011

Type	Article PeerReviewed

Format	application/pdf

Language	en

Identifier	http://ir.cftri.com/10127/1/Process_Biochemistry%2C_Volume_46%2C_Issue_2%2C_February_2011%2C_Pages_458-464.pdf Devaraj, K. B. and Ramesh Kumar, Parigi and Prakash, V. (2011) Comparison of activity and conformational changes of ficin during denaturation by urea and guanidine hydrochloride. Process Biochemistry, 46. pp. 458-464.