Expression profile of phenylpropanoid pathway genes in Decalepis hamiltonii tuberous roots during flavour development.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/14092/
https://doi.org/10.1007/s13205-018-1388-7 |
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| Title |
Expression profile of phenylpropanoid pathway genes in Decalepis
hamiltonii tuberous roots during flavour development.
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| Creator |
Kiran, K.
Priyanka, P. S. Veeresh, Lokesh Nandini, P. Shetty Giridhar, P. |
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| Subject |
15 Flavour/Fragrance/Perfumes
23 Vegetables |
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| Description |
To explore the transcriptional control of phenylpropanoid pathway (PPP) involved in vanillin flavour metabolites production
in tuberous roots of Decalepis hamiltonii, four PPP key genes expressed during the tuber development were identified and
their mRNA expression profiles were evaluated using quantitative real-time PCR. Flavour metabolite quantification by HPLC
analysis confirmed 10, 170 and 500 μg/g 2-hydroxy-4-methoxy benzaldehyde and 4, 20 and 40 μg/g vanillin in first- (3-monthold
plant), second- (18-month-old plant) and third-stage tubers (60-month-old matured plant), respectively. The expression of
all four genes phenylalanine ammonia lyase (DhPAL), cinnamate-4-hydroxylase (DhC4H), caffeic acid-O-methyltransferase
(DhCOMT) and vanillin synthase (DhVAN) increased with flavour development from first stage to second stage. A decrease
in expression from 1.9-folds to 0.1-folds and 19.2-folds to 5.2-folds for DhCOMT and DhVAN was recorded for second stage
to third stage, respectively. However, a gradual increase in expression of DhPAL (up to 26.4-folds) and a constant expression
pattern for DhC4H (up to 7.1-folds) was evident from second stage to third stage of flavour development. The decrease in
the expression levels of DhCOMT and DhVAN in third stage shows that the second-stage tubers are more transcriptionally
active towards flavour biosynthesis.
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| Date |
2018
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/14092/1/3%20Biotech%20%282018%29%208365.pdf
Kiran, K. and Priyanka, P. S. and Veeresh, Lokesh and Nandini, P. Shetty and Giridhar, P. (2018) Expression profile of phenylpropanoid pathway genes in Decalepis hamiltonii tuberous roots during flavour development. 3 Biotech, 8. pp. 1-11. |
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