Genetic Variation of pln Loci Among Probiotic Lactobacillus plantarum Group Strains with Antioxidant and Cholesterol-Lowering Ability.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/14124/
https://doi.org/10.1007/s12602-017-9336-0 |
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| Title |
Genetic Variation of pln Loci Among Probiotic Lactobacillus
plantarum Group Strains with Antioxidant
and Cholesterol-Lowering Ability.
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| Creator |
Sundru, Manjulata Devi
Prakash, M. Halami |
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| Subject |
03 Biochemistry & Molecular Biology
02 Bacteriology |
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| Description |
In the present study, 14 different plantaricinencoding
genes of pln loci were studied and compared to
available sequences from public domain database of probiotic
Lactobacillus plantarum strains. Based upon the presence and
absence of selected genes, pln locus was grouped into eight
clusters. Further, quantitative real-time PCR (qRT-PCR) analysis
for seven genes has discriminated the complex pln locus
into five types which includes WCFS1 (in Lactobacillus
plantarum subsp. plantarum MCC 2976 and MCC 2974 and
Lactobacillus paraplantarum MCC 2978), closely related to
J51 (in Lb. paraplantarum MCC 2973 and MCC 2977), J23
(in Lb. plantarum MTCC 5422), NC8 (in Lb. paraplantarum
MTCC 9483), and a new E1 type (in Lb. plantarum subsp.
plantarum E1). It was observed that the plnA, EF, NC8βα,
NC81F, NC8HK, and G were expressed in E1 strain. Further,
southern hybridization confirmed the chromosome-encoded
plantaricin in Lb. plantarum group (LPG) strains. Several
PCR assays and DNA sequence analysis of the regions amplified
in pln loci of E1 isolate suggested a hybrid variant of
NC8 and J51 plantaritypes. This indicates the wide distribution
of plantaricin with remarkable variation, diversity, and
plasticity among the LPG strains of vegetable origin.
Further, the selected strains were able to reduce the growth
of Kocuria rhizophila ATCC 9341 by 40–54% within 6 h of
co-incubation under in vitro pathogen exclusion assay. These
isolates also possessed cholesterol-lowering and antioxidant
activity suggesting their application in the development of
functional foods.
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| Date |
2019
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/14124/1/Probiotics%20%26%20Antimicro.%20Prot.%20%282019%29%201111%E2%80%9322.pdf
Sundru, Manjulata Devi and Prakash, M. Halami (2019) Genetic Variation of pln Loci Among Probiotic Lactobacillus plantarum Group Strains with Antioxidant and Cholesterol-Lowering Ability. Probiotics and Antimicrobial Proteins, 11. pp. 11-22. |
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