Synergistic method for extraction of high purity Allophycocyanin from dry biomass of Arthrospira platensis and utilization of spent biomass for recovery of carotenoids.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/14134/
https://doi.org/10.1016/j.seppur.2019.05.064 |
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| Title |
Synergistic method for extraction of high purity Allophycocyanin from dry biomass
of Arthrospira platensis and utilization of spent biomass for recovery of
carotenoids.
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| Creator |
Hrishikesh, A. Tavanandi
Prasad, V. Raghavarao, K. S. M. S. |
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| Subject |
14 Carotenoid Chemistry
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| Description |
Allophycocyanin (A-PC) and carotenoids are high value low volume compounds
present in Arthrospira platensis which are usually ignored and left un-extracted (50-
80%) in the spent biomass after extraction of C-Phycocyanin. In the present work,
process parameters such as incubation time, solid-liquid (S/L) ratio and
surfactant/enzyme concentration was standardized for surfactant and enzyme-assisted
extraction of A-PC. Enzyme-assisted method resulted in very high A-PC purity (1.19)
with a yield of 32.27 mg/g dry biomass (db). To improve the yield, enzyme-assisted
method was combined with ultrasonication which resulted in the highest yield of 44.08
mg/g (db) with a purity of 1.09 (the highest reported till date during primary extraction).
The spent biomass after Low-Humidity (LH) drying was used for the extraction of
carotenoids and the best conditions were 80% concentration of ethanol, 1:4 S/L ratio,
pH 7, 40 oC temperature and 1 h extraction time. LH drying was observed to be a
possible alternative to freeze drying for drying of spent biomass.
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| Date |
2019
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/14134/1/Separation%20and%20Purification%20Technology%202019.pdf
Hrishikesh, A. Tavanandi and Prasad, V. and Raghavarao, K. S. M. S. (2019) Synergistic method for extraction of high purity Allophycocyanin from dry biomass of Arthrospira platensis and utilization of spent biomass for recovery of carotenoids. Separation and Purification Technology, 225. pp. 97-111. ISSN 1383-5866 |
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