Heterogeneity of macrolide-lincosamide-streptogramin phenotype & conjugal transfer of erm(B) in Pediococcus pentosaceus.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/14137/
https://doi.org/10.4103/ijmr.IJMR_2055_17 |
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| Title |
Heterogeneity of macrolide-lincosamide-streptogramin phenotype &
conjugal transfer of erm(B) in Pediococcus pentosaceus.
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| Creator |
Surya Chandra Rao, Thumu
Prakash, M. Halami |
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| Subject |
02 Bacteriology
04 Fermentation Technology |
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| Description |
Background & objectives: Pediococcus pentosaceus has been reported to cause clinical infections while it
is being promoted as probiotic in food formulations. Antibiotic resistance (AR) genes in this species are
a matter of concern for treating clinical infections. The present study was aimed at understanding the
phenotypic resistance of P. pentosaceus to macrolide-lincosamide-streptogramin B (MLSB) antibiotics
and the transfer of AR to pathogens.
Methods: P. pentosacues isolates (n=15) recovered from fermented foods were screened for phenotypic
resistance to MLSB antibiotics using disc diffusion and microbroth dilution methods. Localization and
transferability of the identified resistance genes, erm(B) and msr(C) were evaluated through Southern
hybridization and in vitro conjugation methods.
Results: Four different phenotypes; sensitive (S) (n=5), macrolide (M) (n=7), lincosamide (L) (n=2) and
constitutive (cMLSB) (n=1) were observed among the 15 P. pentosaceus isolates. High-level resistance (>256
μg/ml) to MLSB was observed with one cMLSB phenotypic isolate IB6-2A. Intermediate resistance (8-16
μg/ml) to macrolides and lincosamides was observed among M and L phenotype isolates, respectively.
Cultures with S phenotype were susceptible to all other antibiotics but showed unusual minimum
inhibitory concentration (MIC) values of 8-16 μg/ml for azithromycin. Southern hybridization studies
revealed that resistance genes localized on the plasmids could be conjugally transferred to Enterococcus
faecalis JH2-2.
Interpretation & conclusions: The study provides insights into the emerging novel resistance patterns in
P. pentosaceus and their ability to disseminate AR. Monitoring their resistance phenotypes before use of
MLS antibiotics can help in successful treatment of Pediococcal infections in humans.
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| Date |
2019
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/14137/1/IndianJMedRes1492270-2451763_064837.pdf
Surya Chandra Rao, Thumu and Prakash, M. Halami (2019) Heterogeneity of macrolide-lincosamide-streptogramin phenotype & conjugal transfer of erm(B) in Pediococcus pentosaceus. Indian Journal of Medical Research, 149. pp. 270-275. |
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