Presence of extracellular DNA & protein in biofilm formation by gentamicin-resistant Lactobacillus plantarum.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/14138/
https://doi.org/10.4103/ijmr.IJMR_2022_17 |
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| Title |
Presence of extracellular DNA & protein in biofilm formation by
gentamicin-resistant Lactobacillus plantarum.
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| Creator |
Jaimee, George
Prakash, M. Halami |
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| Subject |
03 Biochemistry & Molecular Biology
02 Bacteriology |
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| Description |
Background & objectives: Bacterial biofilms a multi-layered defence, comprise extracellular DNA (eDNA)
and proteins, protect bacteria from harmful environment and nutrient limitation and utilize the mutual
benefits within a community. Bacterial biofilms also defend bacteria from harsh environments such as
antibiotic treatment. This leads to poor antibiotic penetration, slow growth, adaptive stress responses,
and formation of persister cells. This study was done to determine the relation of antibiotic resistance
deciphered by the biofilms in Lactobacillus plantarum, a lactic acid bacteria (LAB) with probiotic
significance.
Methods: The gentamicin-resistant L. plantarum isolates were allowed to form biofilms and subjected
to DNase I and proteinase K treatment. The optical density (OD) values were recorded for the biofilm
assay and the cell count for the number of viable cells was taken for the control and the test samples.
Percentage reduction was calculated based on the difference between the initial and final OD for both
the parameters.
Results: The biofilm assay revealed that the native L. plantarum isolates which were phenotypically
susceptible, possessed the ability to form biofilms. The OD values were significantly decreased in
comparison to the biofilm-forming control culture when these were treated with DNase I and proteinase K.
Interpretation & conclusions: The study revealed that the biofilms formed by L. plantarum comprised of
eDNA and proteins which was evidenced by the reduction in OD values and percentage in comparison to
the control upon DNase I and proteinase K treatment. This indicates that the eDNA and biofilm matrix
proteins are vital constituents of biofilms and may carry significant risk when coupled with antibiotic
resistance.
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| Date |
2019
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/14138/1/IndianJMedRes1492257-2458559_064945.pdf
Jaimee, George and Prakash, M. Halami (2019) Presence of extracellular DNA & protein in biofilm formation by gentamicin-resistant Lactobacillus plantarum. Indian Journal of Medical Research, 149. pp. 257-262. |
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