Strain-Specific Quantification of Native Probiotic Bacillus spp. and Their Effect on Liver Function and Gut Microflora of Experimental Rats.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/14204/
https://doi.org/10.1007/s12602-018-9391-1 |
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| Title |
Strain-Specific Quantification of Native Probiotic Bacillus spp. and Their
Effect on Liver Function and Gut Microflora of Experimental Rats.
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| Creator |
Shobharani, P.
Muthukumar, S. P. Dhanya, K. Prakash, M. Halami |
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| Subject |
17 Fatty Acid Chemistry
02 Bacteriology |
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| Description |
Safety and efficacy was investigated for two candidate probiotic B. flexus MCC2427 and B. licheniformis MCC2512 via in vivo
studies on albinoWistar rats. In acute toxicity assay, rats were fed with single dose of 1010 cfu mL−1 of probiotics. The follow-up
studies for next 14 days did not reveal any toxicity-related criteria indicating the non-toxicity nature of probiotics. In 90-day
repeated dosage studies, the cultures were administered in three doses (106, 107, 108 cfu mL−1). Results showed no overt toxic
effect and no drastic treatment-related changes pertaining to histopathology of vital organs. DNA fingerprinting indicated the lack
of bacterial translocation. Superoxide dismutase and catalase activity indicated their antioxidant potential. Reduced serum
cholesterol with improved HDL-cholesterol specified the cholesterol-reducing ability of the cultures, which was also apparent
with increased excretion of cholic acid in feces. Both probiotic cultures positively altered the gut microbial environment, retained
lactic acid bacterial effect, and simultaneously reduced pathogenic strains. A sensitive and rapid tool was developed using strainspecific
qPCR primers, which facilitated appropriate estimation of test culture in feces. The data strongly advocate the safety of
tested probiotics at levels used in the study.
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| Date |
2019
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/14204/1/Probiotics%20and%20Antimicrobial%20Proteins%20%282019%29%2011478%E2%80%93492.pdf
Shobharani, P. and Muthukumar, S. P. and Dhanya, K. and Prakash, M. Halami (2019) Strain-Specific Quantification of Native Probiotic Bacillus spp. and Their Effect on Liver Function and Gut Microflora of Experimental Rats. Probiotics and Antimicrobial Proteins, 11. pp. 478-492. |
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