Low Molecular Weight Chitosan (∼20 kDa) protects acrylamide induced oxidative stress in D. melanogaster by restoring dopamine and KIF5B levels.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/14207/
https://doi.org/10.1016/j.carbpol.2019.115005 |
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| Title |
Low Molecular Weight Chitosan (∼20 kDa) protects acrylamide induced
oxidative stress in D. melanogaster by restoring dopamine and KIF5B levels.
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| Creator |
Pramod Kumar, P.
Harish Prashanth, K. V. |
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| Subject |
17 Toxicology
18 Processed foods |
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| Description |
Acrylamide (AA) presence and formation are predominant in fried, baked and heat-processed foods. Using
Drosophila model, we have investigated the dietary AA-arbitrate oxidative stress induced neurotoxicity and the
effect of soluble Low Molecular Weight Chitosan (LMWC) supplementation. We assessed the neurodegenerative
and behavioural effect of AA (0–10 mM) exposure in Drosophila (adult males). As a result, the exposed flies
showed distinctive locomotor impairments and incident of mortality [51% in 5mM AA (sub-toxic level) for 7
days] and higher mortality with increased concentration of acrylamide. Further, exposure of AA toxicity was also
correlated with changing levels of oxidative markers, ETC complexes and cholinergic function of flies. Decreased
dopamine (25 μg/mg) and kinesin motor protein levels were confirmed by HPLC and Immunoblotting studies,
respectively. Interestingly, the co-exposure of LMWC alongside AA ameliorates respective biochemical changes
with restoring dopamine (30 μg/mg, control groups 32 μg/mg) and kinesin motor protein (KIF5B) levels. These
results indicated that supplementation of biocompatible LMWC may be promising candidate for complete protection
against AA induced oxidative stress.
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| Date |
2019
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/14207/1/Carbohydrate%20Polymers%20222%20%282019%29%20115005.pdf
Pramod Kumar, P. and Harish Prashanth, K. V. (2019) Low Molecular Weight Chitosan (∼20 kDa) protects acrylamide induced oxidative stress in D. melanogaster by restoring dopamine and KIF5B levels. Carbohydrate Polymers, 222. pp. 1-10. |
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