CSIR Central

Development and validation of headspace Solid-Phase microextraction coupled with gas chromatography (HSSPME-GC) method for the analysis of zingiber zerumbet L.

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore


Field	Value

Relation	http://ir.cftri.com/14224/ https://doi.org/10.1080/14786419.2019.1643863

Title	Development and validation of headspace Solid-Phase microextraction coupled with gas chromatography (HSSPME-GC) method for the analysis of zingiber zerumbet L.

Creator	Bhavya, M. L. Ravi, R. Madhava Naidu, M.

Subject	01 Analysis

Description	Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography (GC-FID) was explored to determine the fingerprinting characteristic of Zingiber zerumbet L. volatiles to differentiate between different ginger species. The effect of different fibers [polydimethylsiloxane (PDMS, 7lm), polyacrylate (PA, 85lm)], different temperature and time on the HS-SPME-GC was investigated by using response surface methodology coupled with full factorial experimental design. The area percentage of the major sesquiterpenes (Zerumbone and a-humulene) were 56.3±4.7% and 47.5±27.2% with PA fiber, respectively at optimum condition of 70�C and 30min. ValidationofthedevelopedHS-SPME-GCmethodwithlimitsofdetection and quantification for zerumbone was 0.09 and 0.28lg/g, respectively, demonstrating the suitable sensitivity of HS-SPME-GC methodforthequantificationofsesquiterpenes.Therefore,thesimplicity of HS-SPME-GC makes it a convenient tool for qualitative and quantitative comparison of different ginger species targeting at the markersesquiterpenemolecule,zerumbone.

Date	2019

Type	Article PeerReviewed

Format	pdf

Language	en

Identifier	http://ir.cftri.com/14224/1/NATURAL%20PRODUCT%20RESEARCH.pdf Bhavya, M. L. and Ravi, R. and Madhava Naidu, M. (2019) Development and validation of headspace Solid-Phase microextraction coupled with gas chromatography (HSSPME-GC) method for the analysis of zingiber zerumbet L. Natural Product Research.