Transcription analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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| Relation |
http://ir.cftri.com/14229/
https://doi.org/10.1016/j.fm.2019.103336 |
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| Title |
Transcription analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1.
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| Creator |
Deepesh, Panwar
Mukesh, Kapoor |
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| Subject |
09 Food Microbiology
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| Description |
Plantderivedgalactomannooligosaccharides(GMOS)areanemergingclassofprebiotics,butnoinformationis available on theirutilization in lactobacilli atthe molecular level. Thecurrent studyaimed atidentifying the geneticlociinvolvedinthetransportandcatabolismoflocustbeangumderivedGMOSinLactobacillusplantarum WCFS1. Substrate depletion study showed that L. plantarum WCFS1 can metabolize only short chain GMOS (degreeofpolymerization;DP≤3).GlobaltranscriptomemicroarrayprofilingofL.plantarumWCFS1revealed differential expression when GMOS or control sugars (glucose, galactose, and mannose) were used as a sole carbohydratesource.Twogeneticlociinvolvedincellobiose(~3.2kb)andoligo-sucrose(~7.3kb)utilizationin L. plantarumWCFS1werehighlyup-regulatedupto8.3 andupto6.7-fold,respectivelyby GMOSutilization. qRT-PCR studies of the selected gene clusters showed correlation with microarray data. Altogether, transcriptomeandqRT-PCRstudiesofL.plantarumWCFS1suggestedthatun-substitutedmannobiose(DP2)mightbe metabolized by proteins encoded by the cellobiose operon while, substituted DP2 (galactomannose) and DP3 (galactomannobiose)weremostlikelytransportedandcatabolizedbytheoligo-sucroseutilizationlociencoded proteins.
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| Date |
2020
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/14229/1/Food%20Microbiology%2086%20%282022%29%20103336.pdf
Deepesh, Panwar and Mukesh, Kapoor (2020) Transcription analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1. Food Microbiology, 86. pp. 1-8. |
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