CSIR Central

OsPLB gene expressed during seed germination encodes a phospholipase in rice.

IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore


Field	Value

Relation	http://ir.cftri.com/14279/ https://doi.org/10.1007/s13205-019-2016-x

Title	OsPLB gene expressed during seed germination encodes a phospholipase in rice.

Creator	Achintya Kumar, Dolui Latha, M. Vijayaraj, P.

Subject	05 Enzymes 01 Rice

Description	Hydrolysis of phospholipid monolayer by phospholipases is an important event in the mobilization of stored lipids for seed germination. However, the identification and functional characterization of cereal phospholipases, especially during rice germination, are limited. In the present study, we have identified and characterized a phospholipase OsPLB gene expressed during germination. The full-length coding region of OsPLB was cloned into pRSETA as well as pYES2/NTC vector. The recombinant protein was successfully expressed in both E. coli and Saccharomyces cerevisiae. The recombinant protein was purified to homogeneity by affinity chromatography, and it was further confirmed by MS/MS analysis. In vitro lipase assay and lipidome analysis using high-resolution mass spectrometry showed phosphatidylcholine (PC) specific phospholipase B activity. The results revealed that protein encoded by OsPLB gene prefers to hydrolyze PCs with C28, C32, and C34 containing unsaturated fatty acids. Collectively, the present study describes the identification and characterization of a phospholipase B, which hydrolyze PC, a major component of phospholipid monolayer covering storage lipid, as an initial event during rice seed germination.

Date	2020

Type	Article PeerReviewed

Format	pdf

Language	en

Identifier	http://ir.cftri.com/14279/1/3%20Biotech%20%282020%29%201030.pdf Achintya Kumar, Dolui and Latha, M. and Vijayaraj, P. (2020) OsPLB gene expressed during seed germination encodes a phospholipase in rice. 3 Biotech, 10. pp. 1-9.