Edible plant-derived exosomal microRNAs: Exploiting a cross-kingdom regulatory mechanism for targeting SARS-CoV-2.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/14799/
https://doi.org/10.1016/j.taap.2021.115425 |
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| Title |
Edible plant-derived exosomal microRNAs: Exploiting a cross-kingdom
regulatory mechanism for targeting SARS-CoV-2.
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| Creator |
Sreeram, P. K.
Gopinath, M. S. |
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| Subject |
10 Plants
03 Biochemistry & Molecular Biology |
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| Description |
Background: The current COVID-19 pandemic is caused by SARS-CoV-2 which belongs to coronaviridae family.
Despite the global prevalence, there are currently no vaccines or drugs. Dietary plant derived exosome-like
vesicles are known as edible nanoparticles (ENPs). ENPs are filled with microRNAs (miRNAs), in bioavailable
form. Recently, cross-kingdom regulation of human transcripts by plant miRNAs have been demonstrated.
However, ENP derived miRNAs targeting SARS-CoV-2 has not been described.
Study design: Mature ENP-derived miRNA sequences were retrieved from small RNA sequencing datasets available in the literature. In silico target prediction was performed to identify miRNAs that could target SARS-CoV-2.
ENPs were isolated from ginger and grapefruit plants and the expression of SARS-CoV-2 targeting miRNAs were
confirmed by qRT-PCR.
Results: From a total of 260 ENP-derived miRNAs, we identified 22 miRNAs that could potentially target SARSCoV-2 genome. 11 miRNAs showed absolute target specificity towards SARS-CoV-2 but not SARS-CoV. ENPs
from soybean, ginger, hamimelon, grapefruit, tomato and pear possess multiple miRNAs targeting different regions within SARS-CoV-2. Interestingly, osa/cme miR-530b-5p specifically targeted the ribosomal slippage site
between ORF1a and ORF1b. We validated the relative expression of six miRNAs (miR-5077, miR-6300, miR156a, miR-169, miR-5059 and miR-166 m) in ginger and grapefruit ENPs by RT-PCR which showed differential
enrichment of specific miRNAs in ginger and grapefruit ENPs.
Conclusion: Since administration of ENPs leads to their accumulation into lung tissues in vivo, ENP derived
miRNAs targeting SARS-CoV-2 genome has the potential to be developed as an alternative therapy.
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| Date |
2021
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/14799/1/Toxicology%20and%20Applied%20Pharmacology%20414%20%282021%29%20115425.pdf
Sreeram, P. K. and Gopinath, M. S. (2021) Edible plant-derived exosomal microRNAs: Exploiting a cross-kingdom regulatory mechanism for targeting SARS-CoV-2. Toxicology and Applied Pharmacology, 414. p. 115425. |
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