Acid and Pepsin Soluble Collagens from Skin By-product of Red-bellied Pacu (Piaractus brachypomus): Extraction and Comparative Characterizations Towards Finding Substitute to Bovine and Porcine Collagen.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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Relation |
http://ir.cftri.com/14804/
https://doi.org/10.1080/10498850.2021.1884374 |
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Title |
Acid and Pepsin Soluble Collagens from Skin
By-product of Red-bellied Pacu (Piaractus
brachypomus): Extraction and Comparative
Characterizations Towards Finding Substitute to
Bovine and Porcine Collagen.
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Creator |
Lidiya, C. Johny
Tanaji, G. Kudre Suresh, P. V. |
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Subject |
03 Proteins
Fish |
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Description |
This investigation focused on the sustainable valorization of red-bellied pacu
(RBP) skin towards finding an alternative to bovine and porcine collagen.
Acid-soluble collagen (ASC, 45.8 ± 0.7%) and pepsin soluble collagen (PSC,
57.8 ± 1.9%) were successfully extracted from RBP skin and comparatively
elucidated their characteristics and in-vitro fibril-forming potential. The
extracted collagens were characterized as type I collagen and confirmed
the well-preserved triple-helical configuration by sodium dodecyl sulphate
polyacrylamide gel electrophoresis and Fourier transform-infrared spectro�scopy analysis. Both ASC and PSC exhibited relatively better thermo-stability,
and the denaturation temperatures were found to be 35.5–35.29 °C by
differential scanning calorimetry analysis. Both collagens displayed higher
(p ≤ 0.05) solubility at low NaCl concentrations. The extracted ASC and PSC
showed the same degree of fibril-forming capacities and confirmed well�defined fibril morphologies. Overall, the results showed the possibilities of
effectively exploring RBP skin as a potential alternative source for realistic
and safe collagen for food, nutrition, pharmaceutical, and other applications.
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Date |
2021
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Type |
Article
PeerReviewed |
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Format |
pdf
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Language |
en
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Identifier |
http://ir.cftri.com/14804/1/JOURNAL%20OF%20AQUATIC%20FOOD%20PRODUCT%20TECHNOLOGY%202021.pdf
Lidiya, C. Johny and Tanaji, G. Kudre and Suresh, P. V. (2021) Acid and Pepsin Soluble Collagens from Skin By-product of Red-bellied Pacu (Piaractus brachypomus): Extraction and Comparative Characterizations Towards Finding Substitute to Bovine and Porcine Collagen. Journal of Aquatic Food Product Technology. ISSN ISSN: 1049-8850 |
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