The Synergistic role of serotonin and melatonin during temperature stress in promoting cell division, ethylene and isoflavones biosynthesis in Glycine max.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.res.in/14862/
https://doi.org/10.1016/j.cpb.2021.100206 |
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| Title |
The Synergistic role of serotonin and melatonin during temperature stress
in promoting cell division, ethylene and isoflavones biosynthesis in
Glycine max.
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| Creator |
Gyanendra, Kumar
Kirti, R. Saad Monisha, Arya Bijesh, Puthusseri |
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| Subject |
10 Plants
02 Plant Biochemistry |
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| Description |
Serotonin (SER) and melatonin (MEL) are signalling molecules involved in signalling in various stress response
mechanisms in plants. However, their role and molecular mechanism in the accumulation of isoflavones and
biomass under temperature stress are not clearly defined. To explicate their function, SER and MEL at different
ratios were applied to the in vitro cultures of soybean at 16, 24, and 32 ◦C and were analyzed for the accumulation
of bioactive compounds, isoflavones content, and culture biomass followed by transcript levels of cell
division, isoflavones, and ethylene biosynthesis genes. An increase in biomass, total phenolics, total flavonoids,
and different forms of isoflavones content was evident in the treatments. The expression level of critical genes of
isoflavone (chalcone synthase, chalcone reductase, chalcone isomerase, and isoflavone synthase), ethylene (s-adenosyl
methionine and 1-aminocyclopropane-1-carboxylic acid oxidase), cell division (cyclin and cyclin-dependent kinase),
and MEL biosynthesis genes (acetyl serotonin O-methyltransferase and serotonin N-acetyltransferase) and transcription
factors (melatonin 2 hydroxylase, myeloblastosis like protein) were co-expressed during temperature stress
in the presence of SER and MEL. At 32 ◦C, the SER and MEL application showed maximum biomass and isoflavones
content and was substantiated with the corresponding gene expression. We hypothesize that the SER
and MEL ratio may play a role in increasing the biomass and isoflavones content under temperature stress in
soybean cell culture.
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| Date |
2021
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.res.in/14862/1/Current%20Plant%20Biology%2026%20%282021%29%20100206.pdf
Gyanendra, Kumar and Kirti, R. Saad and Monisha, Arya and Bijesh, Puthusseri (2021) The Synergistic role of serotonin and melatonin during temperature stress in promoting cell division, ethylene and isoflavones biosynthesis in Glycine max. Current Plant Biology, 26. p. 100206. |
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