Digital image processing-an alternate tool for monitoring of pigment levels in cultured cells with special reference to green alga Haematococcus pluvialis.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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http://ir.cftri.com/1966/
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| Title |
Digital image processing-an alternate tool for monitoring of pigment levels in cultured cells with special reference to green alga Haematococcus pluvialis.
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| Creator |
Sandesh Kamath, B.
Ms, Shalini Chidambar Brinda, B. R. Kumar, M. A. Sarada, R. Ravishankar, G. A. |
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| Subject |
11 Food Biochemistry
08 Food Chemistry |
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| Description |
A method for analyzing carotenoid content in Haematococcus pluvialis, a green alga was developed using digital image processing (DIP) and an artificial neural network (ANN) model. About 90 images of algal cells in various phases of growth were processed with the tools of DIP. A good correlation of R(2)=0.967 was observed between carotenoid content as estimated by analytical method and DIP. Similar correlation was also observed in case of chlorophyll. Since the conventional methods of carotenoid estimation are time consuming and result in loss of pigments during analysis, DIP method was found to be an effective online monitoring method. This method will be useful in measurement of pigments in cultured cells.
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| Date |
2005
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| Type |
Article
NonPeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://ir.cftri.com/1966/1/BiosensBioelectron21768-773.pdf
Sandesh Kamath, B. and Ms, Shalini Chidambar and Brinda, B. R. and Kumar, M. A. and Sarada, R. and Ravishankar, G. A. (2005) Digital image processing-an alternate tool for monitoring of pigment levels in cultured cells with special reference to green alga Haematococcus pluvialis. Biosensors and Bioelectronics, 21. pp. 768-773. ISSN 0956-5663 |
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