Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by cetyltrimethylammonium bromide.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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| Relation |
http://ir.cftri.com/2031/
BL-01-87 |
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| Title |
Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by cetyltrimethylammonium bromide.
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| Creator |
Joshi, M. S.
Gowda, L. R. Bhat, S. G. |
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| Subject |
27 Dairy products
04 Yeast |
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| Description |
Whole cells of lactose-fermenting K. fragilis had very low beta-galactosidase activity. Treating these cells with a cationic detergent, cetyltrimethylammonium bromide (0.1%), at 4 degree C for 5 min increased enzyme activity 480-fold. Detergent-treated cells readily hydrolysed lactose in milk and sweet whey; glucose produced was not metabolized further. Detergent-permeabilized cells could be used, e.g. to produce low lactose milk, or in utilization of whey. [From En summ.]
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| Date |
1987
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/2031/1/Biotechnology%20Letters_1987.pdf
Joshi, M. S. and Gowda, L. R. and Bhat, S. G. (1987) Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by cetyltrimethylammonium bromide. Biotechnology Letters, 9 (8). pp. 549-554. ISSN 0141-5492 |
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