Enhanced biosynthesis of polyhydroxyalkanoates in a mutant strain of Rhizobium meliloti.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/2060/
|
|
| Title |
Enhanced biosynthesis of polyhydroxyalkanoates in a mutant strain of Rhizobium meliloti.
|
|
| Creator |
Lakshman, Kshama
Shamala, Tumkur Ramachandriah |
|
| Subject |
09 Food Microbiology
|
|
| Description |
Strains of Rhizobium spp. isolated from leguminous plants and standard strains accumulated 27% to 57% polyhydroxyalkanoate (PHA) of their cell biomass. Among these cultures, one strain of Rhizobium meliloti synthesized 10-30% more PHA than others and contained 3% hydroxyvalerate (HV) when grown on sucrose as carbon substrate. The occurrence of hydroxybutyrate (HB) and HV was confirmed by GC and 1H NMR analysis. Treatment of the culture with 4'-N-piperidinobutyl-2-chlorophenoxazine resulted in a mutant which synthesized up to 69%, PHA of the cell biomass with an improved yield of 11 to 47% under different carbon and nitrogen ratios, compared to the parent strain.
|
|
| Date |
2003
|
|
| Type |
Article
NonPeerReviewed |
|
| Format |
application/pdf
|
|
| Language |
en
|
|
| Rights |
—
|
|
| Identifier |
http://ir.cftri.com/2060/1/Biotechnol_Letters_2003_25_2_115-119.pdf
Lakshman, Kshama and Shamala, Tumkur Ramachandriah (2003) Enhanced biosynthesis of polyhydroxyalkanoates in a mutant strain of Rhizobium meliloti. Biotechnology Letters, 25 (2). pp. 115-119. ISSN 0141-5492 |
|