Phytase from Citrobacter koseri PM-7: Enhanced production using statistical method and application in ameliorating mineral bioaccessibility and protein digestibility of high-phytate food.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/2376/
https://doi.org/10.1080/10826068.2017.1405024 |
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| Title |
Phytase from Citrobacter koseri PM-7: Enhanced production using statistical method
and application in ameliorating mineral bioaccessibility and protein digestibility
of high-phytate food.
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| Creator |
Preethi, Tripathi
Jyothi Lakshmi, A. Mukesh, Kapoor |
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| Subject |
03 Proteins
05 Enzymes |
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| Description |
The present study was aimed at enhancing phytase (Phy-Ck) production from Citrobacter koseri PM-7
using response surface methodology (RSM) and improving the bioaccessibility of minerals (Fe and Zn)
and protein digestibility in high-phytate food using Phy-Ck. A five-variable and three-level central
composite design of RSM using wheat bran (6.681%, w/v), inoculum level (2.5%, v/v), and triton X-100
(0.2%, v/v) resulted in up to 5.57-fold (1.047 U/ml) improvement in Phy-Ck yield from C. koseri PM-7 when
compared with fermentation media I and II. The model was successfully validated in the design space by
taking a random set of variable combinations. Treatment of high-phytate food with partially purified Phy-
Ck showed improvement in mineral bioaccessibility maximally for defatted sesame flour (DSF) (Fe 45.5%;
Zn 50.7%) followed by wheat flour (WF) (Fe 13.5%; Zn 14.4%), green gram flour (GGF) (Fe 0.7%; Zn 3.8%)
and defatted groundnut flour (DGF) (Zn 5.6%). The in vitro protein digestibility (IVPD) of WF increased
from 48.83 to 65.04%, GGF from 45.04 to 57.12%, and DSF from 47.34 to 55.7% after Phy-Ck treatment.
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| Publisher |
Taylor & Francis
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| Date |
2018
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| Type |
Article
PeerReviewed |
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| Format |
pdf
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| Language |
en
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| Identifier |
http://ir.cftri.com/2376/1/PREPARATIVE%20BIOCHEMISTRY%20AND%20BIOTECHNOLOGY%202018%2C%20VOL.%2048%2C%20NO.%201%2C%2084%E2%80%9391%20%282%29.pdf
Preethi, Tripathi and Jyothi Lakshmi, A. and Mukesh, Kapoor (2018) Phytase from Citrobacter koseri PM-7: Enhanced production using statistical method and application in ameliorating mineral bioaccessibility and protein digestibility of high-phytate food. Preparative Biochemistry and Biotechnology, 48 (1). pp. 84-91. ISSN 1082-6068 |
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