A comparative study on depolymerization of chitosan by proteolytic enzymes.
IR@CFTRI: CSIR-Central Food Technological Research Institute, Mysore
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| Relation |
http://ir.cftri.com/2451/
CP-01-04 |
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| Title |
A comparative study on depolymerization of chitosan by proteolytic enzymes.
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| Creator |
Vishu Kumar, A. B.
Tharanathan, R. N. |
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| Subject |
05 Enzymes
28 Meat, Fish & Poultry |
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| Description |
Proteolytic enzymes such as pepsin, papain and pronase caused depolymerization of chitosan, a co-polysaccharide of glucosamine and
N-acetyl glucosamine residues linked by b-1,4-glycosidic bonds. The pH optima of these enzymes towards chitosanolysis were different
from that towards their own substrates, indicating the involvement of pH-sensitive conformational changes during specific and non-specific
activities. The depolymerization reaction obeyed Michaelis-Menten kinetics and Km and Vmax values indicated higher affinity of pepsin
towards chitosan. The chitosanolytic products were low molecular weight chitosans (LMWC, a major product), chitooligomers (COs) as well
as monomers. Low molecular weight chitosans had molecular weight in the range, 4.1–10.0 kDa depending on the reaction time. FT-IR
indicated a decrease in the degree of acetylation of LMWC. GPC and HPLC of COs showed a degree of polymerization of 2–8 with a
preponderance of di- to hexamer including monomers.
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://ir.cftri.com/2451/1/Carbohydate_Polymers_Volume_58_No_3_275-283.pdf
Vishu Kumar, A. B. and Tharanathan, R. N. (2004) A comparative study on depolymerization of chitosan by proteolytic enzymes. Carbohydrate Polymers, 58 (3). pp. 275-283. |
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